Measurement

Part:BBa_K2163000

Designed by: Rhushikesh Phadke   Group: iGEM16_IIT_Kharagpur   (2016-10-14)


Measurement of Protease Activity using FRET

This part Consists of 3 biobricks, namely Cyan Fluorescent Protein, HIV Cleavage Site and Yellow Fluorescent Protein. The cleavage site is specific to HIV 1 Aspartyl Protease. The two proteins form a FRET pair. FRET stands for Fluorescence Resonance Energy Transfer. It is a phenomenon is which excitation of one protein leads to emission from the other, due to overlapping wavelengths. The two proteins need to be close together for this to take place. In our project we make use of this to measure the protease activity of HIV 1 Aspartyl Protease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1423


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